International Journal of Applied Biology and Forensics

CRISPR/cas9: An Emerging Revolution in Therapeutics

Makiya Aqeel and Ali Raza

Department of Microbiology, University of Karachi, Karachi, Pakistan

Department of Forensic Sciences, University of Veterinary and Animal Sciences, Lahore, Pakistan




Clustered Regular Interspaced Short Palindromic Repeats also known as CRISPR, the most rapidly adopted genome editing tool was discovered through studies on bacterial immunity system. As genome editing and studies related to its functionality requires to manipulate DNA and modify genomes. Already available genome editing tools like HR-mediated targeting, ZFN, TALEN and Cre-lox etc have certain limitation but the challenge had over come by CRISPR combined with cas9 protein. Because of the fact that future of medicinal and therapeutics seems to reside in adjustments at molecular level by genetic modifications and genome editing in cells. Alteration of a gene expression, inhibition of a gene through advanced methodologies of CRISPR/cas can provide insight tothe key cause of many globally recognized diseases. This review is brief overview of highlighted present day exigent assignments of this technology in various medical and biotechnological areas to explore further horizons for therapeutic domain of research.

Keywords: Genome editing, Therapeutic, CRISPR, cas9, ZFN, TALEN