International Journal of Applied Biology and Forensics

Sequencing and Phylogenetic Analysis of Helicobacter pylori through 16S rRNA Gene Isolated from Gastritis Biopsies

Laith Abdul Hassan AL Obaidi and Abeer M. Jabbar

Department of biology, College of Science, Al Muthanna University, Iraq




Analysis of 16S rRNA gene sequences is one of the most common methods for investigating the phylogeny and taxonomy of bacteria. The aim of this study is to compare Iraqi H. pylori isolates with isolates from different geographical locations. Total of 45 isolates from males and females with gastritis in Samawah and Diwaniya provinces were used to amplify the 16S rRNA gene by PCR then followed by DNA sequencing. The H. pylori 16S rRNA gene was amplified (1471 bp in size) and sequencing. The target sequence has been deposited in the National Center for Biotechnology Information (NCBI) under the accession Number (KP893893). Homology sequence analysis showed high similarity between our sequence with H. acinonychis and H. cetorum (98%) and the lowest similarity between H. pylori and Microcystis aeruginosa (51%). The phylogenetic analysis displayed high correlation between H. pylori and H. acinonychis (a species infected cheetahs and other big cats) at bootstrap value 80%. The cluster containing these two species is nearest from the African out group root M. aeruginosa. This study provided an additional insight into the profile of our 16S rRNA gene and indicates that the Iraqi H.pylori isolates are closely related to a South African isolate.

Keywords: Helicobacter. Pylori, 16S rRNA, Gastritis, Phylogenetic, Gastritis Biopsies