International Journal of Applied Biology and Forensics

Bacteriological and Physiochemical Analysis of Drinking Water from Different Areas of District Gujrat, Pakistan

Asmaa Bashir Ahmed, Mohsin Gulzar Barq and Sidra Javed

Department of Zoology, University of Gujrat, Gujrat, Pakistan

Institute of Agricultural Science, University of Punjab, Lahore, Pakistan

Correspondence: Asma4494@gmail.com

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Abstract

The contribution of water in causing water borne bacterial infections is continuously neglected and much focus is made on treating infections rather than preventing infections. The human body is easily exposed to rich bacterial diversity of water and it necessitates its bacteriological and physiological analysis to made awareness about its careful use. The quality of water is deteriorating very rapidly due to number of factors.Drinking water samples from different areas of district Gujrat, Pakistan were subjected to bacteriological and physiochemical analysis. In addition to physical parameters like pH, presence of foul odor and suspended particles, temperature of samples, the potential of water to cause infection was also analyzed by studying microbial count. Morphological attributes such as color, shape, surface, elevation and opacity of bacterial colonies were observed. Gram staining and biochemical tests (oxidase test and catalase test) on pure bacterial cultures isolated from drinking water samples, exposed the presence of Staphylococcus, Bacillus,Streptococcus, Streptomyces, Escherichia, Enterococcus and Citrobacter. Furthermore, the bio film forming capacity of the isolated species was also estimated to establish its contribution in inducing infections in the local community. Growths of these strains on Congo red supplemented nutrient agar have shown their bio film forming ability and represented different morphotypes including rough dry and red (rdar) and smooth and white (saw) due to binding expression of Congo red dye with extracellular components of bacterial strains. Citrobacter and Escherichia were present in 37.5% samples and 62.5% water samples contain Staphylococcus strains. Bio film forming characteristic is shown by all isolated bacterial strains.

Keywords: Bacteriological, Physiochemical, Drinking Water, Morphotypes, Staphylococcus