Mouna Akeel Hamed Al-Oebady
College of Science, Al-Muthanna University, Iraq
A total of 150 samples (75 diabetic and 75 non-diabetic) of different ages and both genders (86 male and 64 female) were collected from patients suffering from wound infections who attending the Samawah Hospital of AL-Muthanna governorates through the period of one year (December 2015 – December 2016). The isolation and identification methods of bacterial isolates were followed upon the morphological, cultural and biochemical characteristics in addition to the confirmatory systems such as CHROM agar and Api 20 for differentiation among bacterial species. The phenotypic results showed that the isolation percentage of Propionibacterium granulosumas an anaerobic bacteria was 22(18.6%) as predominant pathogens in diabetic wound followed by Staphylococcus aureus as aerobic bacteria 17(14.4%). While Pseudomonas aeruginosa was 17(15.7%) a predominant pathogens isolated from non-diabetic wound infection. Isolated culture was treated with antibiotics and Amoxycillin was found as the best effective antibiotic which gave (16mm) inhibition zone in comparison with other standard antibiotics.
Key words: Bacterial Isolation, Diabetics, Antibiotics. CHROM agar, APi 20, Wound Infections